Description
Recombinant NPC-specific antigens are bound onto the microwells. Antibodies that are specific to these antigens, if present in diluted human serum, will bind to the antigens on the solid phase, forming antigen-antibody complexes. A combination of the 4 main polypeptides is used. This combination of the polypeptides has shown to be broadly reactive with NPC patient serum. Any excess antibody is removed through washing. Anti-human IgA conjugated with horseradish peroxidase then binds to the antigen-antibody complexes. Excess conjugate is removed by washing. Substrate Developer TMB (tetramethylbenzidine) forms a blue colour in the presence of the bound conjugate